I'm looking at services like 23andme, and see that they identify a wide variety of genetic-based ris…
The main paper for the Plasmodium palciparum genome project (Gardner et al., 2002) repeatedly mentio…
What are the main differences between shotgun sequencing and clone based sequencing?
One way to assemble fragments produced by DNA sequencing (often called reads) is to seek for the sho…
I have a vector sequence and would like to find the following nucleotide sequence in it. AASYWSRA …
Y adapters different sequences to be annealed to the 5' and 3' ends of each molecule in a library. …
I am familiar with Sanger sequencing, but at the level of an undergraduate. A lecturer of mine tried…
During Illumina sequencing there is a step called bridge amplification by which DNA is amplified by …
I have been trying to learn some basic DNA sequencing techniques and have been using BLAST as a refe…
Is that correct that a complete DNA sequencing (the whole genome) need only to be done once (per per…
My apologies if my question is too basic, and please point me to a more appropriate forum. I am rea…
I would like to know what are the options for an average person in a developing country to get acces…
I have to do a task for a university task and I need to understand some things before figuring out h…
As an example, this Scientific American article describes a known area in the dog genome that metabo…
I have a question I would like to pose to the community. I have recently received access to a bench-…
So I have a validated set of primers for rtPCR from Biorad that contains SYBR green. If I do rtPCR,…
In class of molecular biology was posed this question: "How many reading frames start in sequence o…
I've heard about services like 23andme, which offer genetic testing to the general public. As a pers…
I've started teaching myself about next-generation sequencing in preparation for a new job, and I'm …
Is there any method to do pulldown enrichment of DNA breakpoints from a cell? I have found this pape…
The MIBBI and other organizations compile create dozens of "Minimal information about..." guidelines…
There are several companies (most notably 23andMe) providing SNP genotyping for individuals claiming…
After sending a DNA sample for sequencing, the resulting sequence had N's in the beginning and end o…
I am in the process of assembling and annotating the genome of a non-model organism, using almost ex…
I would like to measure the surface density of Staphylococcus bacteria and Rhinoviruses (only those …
I'm reading over the ENCODE Nature papers, and one of the papers referred to is "Global mapping of p…
How might I go about amplifying a gene transcript (mRNA) from animal tissue of which little is known…
Using the Illumina platform, it is cheap and (relatively) easy to sequence huge amounts of DNA or RN…
I'm comparing 12 Metagenomes. I'm using HMM counts of a number of proteins known to exists as groups…
With which bacterial infection in humans has it been shown that bacterial DNA can be found in the bl…
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